IgG from Dermatophagoides pteronyssinus (Der p)-atopic individuals modulates non-atopic thymic B cell phenotype (alfa-4/beta-7) and cytokine production (IFN-γ, IL-9, and IL-10) with direct membrane interaction

• DOI: 10.1038/s41598-024-57950-x
• Publication Date: 2024-03-27T12:02:21Z
• Authors: Daniela Terra de-...; Nicolle Rakanidis...; Beatriz Oliveira ...; Isabella Siuffi B...; Thamires Rodrigue...; Lais Alves do Nas...; Fernando Roberto ...; Marilia Garcia de...; Fábio da-Ressurei...; Carolina Nunes Fr...; Jefferson Russo V...
• Keywords: 0301 basic medicine; B cell; IgG; Dermatophagoides pteronyssinus; Science; Q; R; IL-9; Article; 03 medical and health sciences; IL-22; Medicine; IFN-γ

Abstract Studies about thymic B cells are scarce in the literature, but it was suggested that they can exert modulatory and regulatory functions on immune system. Thymic play some role regulating most frequent allergic background worldwide, atopy induced by mite Dermatophagoides pteronyssinus (Der p). Here, we aimed to evaluate if polyclonal IgG repertoire produced Der p-atopic individuals influence homing cytokine profile of human derived from non-atopic children aged less than seven days. With this purpose, formulations cultivated thymocytes their presence. We also assessed subclasses direct interaction with cell membranes. Our results could demonstrate not reduce expression α4β7 molecule as observed response other frequency IFN-γ- IL-9-producing compared mock condition. induce IL-10-producing control conditions. The failed diminish population IL-13-producing cells, unlike reduction when All had similar levels directly interacted Finally, performed experiments using peripheral where effects were observed. In conclusion, our observation demonstrates modulate potentially generating prone allergy development, which seems occur mature cells.