Advanced fermentation techniques enhance dioxolanone type biopesticide production from Phyllosticta capitalensis

• DOI: 10.1038/s41598-025-92626-0
• Publication Date: 2025-03-07T09:31:01Z
• Authors: Nicolas Reyes Cas...; Jorge Rojas López...; William A. Pachec...; Carmen E. Díaz; M. Fe Andres; Azucena González-...
• Keywords: Biopesticide

In the current work, production of dioxolanone and meroterpene type secondary metabolites under different fermentation conditions by endophytic fungus Phyllosticta capitalensis (isolate YCC4) isolated from leaves endemic plant Persea indica has been evaluated. Different techniques, such as microparticle-enhanced culture (MPEC: bentonite, talcum powder) surface adhesion (SAF: metallic mesh glass wool) were applied to medium. Ethyl acetate extracts fermentations isolate YCC4 analyzed for their content revealed following derivatives: metguignardic acid (1), guignardianone C (2), ethyl guignardate (3), D (4) phenguignardic methyl ester (5), meroterpenes: guignardone A (6) B (7), guignarenone (8), (9) I (10). Additionally, biological activity was tested against aphid Myzus persicae root-knot nematode Meloidogyne javanica. Our study significant variations in target well notable differences biopesticide influenced presence inert supports. Overall, findings indicate that wool (GW) is a high-performance material improve derivatives. These underscore significance innovative cultivation methods MPEC SAF drivers produce valuable fungal organisms.