Salivary apoptotic microvesicles as biomarkers for prognostic non-healing oral ulcers and oral cancer: a cross-sectional study

• DOI: 10.1038/s41598-025-93075-5
• Publication Date: 2025-03-18T10:21:51Z
• Authors: Jian Zhao; Yu Dong; Shuya Shi; Hongqiang Yu; Luanjun Tan
• Keywords: Cross-sectional study; Oral Cancers

Microvesicles (MVs) are membrane vesicles secreted by cells and present in the saliva of healthy individuals. It has various functions been reported to be a biomarker for malignant tumors. The changes levels MVs associated with disease(s) is unclear. This study aimed determine proportion salivary apoptotic their association oral ulcer(s) patients non-healing cancer. Saliva (5 mL) was collected from cancer (at time collection, participant have an cavity lesion; n = 73) volunteers (n 62). A standard differential centrifugation protocol used purification MVs. Dynamic light scattering transmission electron microscopy were characterize Flow cytometry quantify Immunocytochemistry performed according protocol. None smoking drinking habit. majority samples derived more positive fluorescent dye carboxyfluorescein succinimidyl ester than those ulcer(s). Salivary fluid obtained had membrane-limited that round and/or slightly elongated shape, diameters 100–1,000 nm. number higher (p < 0.001). There between degree or severity ulcers Levels elevated confirmed Elevated clinicopathological data Evidence level: IV. Technical efficacy: stage 3.