Proteomic profiling of extracellular vesicles allows for human breast cancer subtyping

• DOI: 10.1038/s42003-019-0570-8
• Publication Date: 2019-09-03T10:08:04Z
• Authors: Stamatia Rontogianni; Eleni Synadaki; Bohui Li; Marte C. Liefaard; Esther H. Lips; Jelle Wesseling; Wei Wu; Maarten Altelaar
• Keywords: Subtyping; Proteome; Extracellular Vesicles; Cancer Biomarkers

Extracellular vesicles (EVs) are a potential source of disease-associated biomarkers for diagnosis. In breast cancer, comprehensive analyses EVs could yield robust and reliable subtype-specific that still critically needed to improve diagnostic routines clinical outcome. Here, we show proteome profiles secreted by different cancer cell lines highly indicative their respective molecular subtypes, even more so than the changes within cells. Moreover, detected evidence biological processes pathways, hyperphosphorylated receptors kinases in connection with disease, compiled set protein signatures closely reflect associated pathophysiology. These unique features revealed our work, replicated material, collectively demonstrate differentiate between subtypes prospect use as non-invasive liquid biopsies diagnosis management patients.