Phase separation of Epstein-Barr virus EBNA2 protein reorganizes chromatin topology for epigenetic regulation

• DOI: 10.1038/s42003-021-02501-7
• Publication Date: 2021-08-16T10:03:52Z
• Authors: Yiting Yang; Xidong Ye; Ranran Dai; Zhaoqiang Li; Yan Zhang; Wei Xue; Yongchang Zhu; Delong Feng; Litao Qin; Xin Wang; Bo Lei; Shixiu Liao; Bingtao Hao
• Keywords: Epigenomics

Abstract Epstein-Barr virus nuclear antigen 2 (EBNA2) is a transactivator of viral and cellular gene expression, which plays critical role in the virus-associated diseases. It was reported that EBNA2 regulates expression by reorganizing chromatin manipulating epigenetics. Recent studies showed liquid-liquid phase separation an essential epigenetic transcriptional regulation. Here we show reorganized topology to form accessible domains (ACDs) host genome separation. The N-terminal region EBNA2, necessary for separation, sufficient induce ACDs. C-terminal domain promotes acetylation regions recruiting histone acetylase p300 According these observations, proposed model its through explain mechanism hijacking controlling