Identification of distinct tumor cell populations and key genetic mechanisms through single cell sequencing in hepatoblastoma

• DOI: 10.1038/s42003-021-02562-8
• Publication Date: 2021-09-08T10:02:24Z
• Authors: Alexander Bondoc; Kathryn Glaser; Kang Jin; Charissa Lake; Stefano Cairo; James Geller; Gregory Tiao; Bruce Aronow
• Keywords: Hepatoblastoma; Identification; Liver tumor

Hepatoblastoma (HB) is the most common primary liver malignancy of childhood, and molecular investigations are limited effective treatment options for chemoresistant disease lacking. There a knowledge gap in investigation key driver cells HB tumor. Here we show single cell ribonucleic acid sequencing (scRNAseq) analysis human tumor, background liver, patient derived xenograft (PDX) to demonstrate gene expression patterns within tumor identify intratumor subtype heterogeneity define differing roles pathogenesis based on intracellular signaling pediatric HB. We have identified cluster by genetic which can be examined mechanism treatments. Identification both critical mechanistic pathways combined with unique populations provide basis discovery novel strategies vitro vivo.