Asymbiotic mass production of the arbuscular mycorrhizal fungus Rhizophagus clarus

• DOI: 10.1038/s42003-021-02967-5
• Publication Date: 2022-01-12T11:02:28Z
• Authors: Sachiko Tanaka; Kayo Hashimoto; Yuuki Kobayashi; Koji Yano; Taro Maeda; Hiromu Kameoka; Tatsuhiro Ezawa; Katsuharu Saito; Kohki Akiyama; Masayoshi Kawaguchi
• Keywords: Rhizophagus irregularis; Strigolactone; Propagule; Obligate

Abstract Arbuscular mycorrhizal (AM) symbiosis is a mutually beneficial interaction between fungi and land plants promotes global phosphate cycling in terrestrial ecosystems. AM are recognised as obligate symbionts that require root colonisation to complete life cycle involving the production of propagules, asexual spores. Recently, it has been shown Rhizophagus irregularis can produce infection-competent secondary spores asymbiotically by adding fatty acid, palmitoleic acid. Furthermore, asymbiotic growth be supported using myristate carbon energy source for their increase fungal biomass. However, spore ability these colonise host roots were still limited compared co-culture fungus with plant roots. Here we show combination two hormones, strigolactone jasmonate, induces large number cultures clarus HR1 presence organic nitrogen. Inoculation asymbiotically-generated promoted plants, observed produced symbiotic culture system. Our findings provide foundation elucidation hormonal control development inoculum schemes.