Comprehensive identification of SWI/SNF complex subunits underpins deep eukaryotic ancestry and reveals new plant components

• DOI: 10.1038/s42003-022-03490-x
• Publication Date: 2022-06-06T10:07:34Z
• Authors: Jorge Hernández-G...; Borja Diego-Martin; Peggy Hsuanyu Kuo; Yasaman Jami-Alah...; Ajay A. Vashisht; James Wohlschlegel; Steven E. Jacobsen; Miguel A. Blázquez; Javier Gallego-Ba...
• Keywords: Multicellular organism; Lineage (genetic); Identification

Over millions of years, eukaryotes evolved from unicellular to multicellular organisms with increasingly complex genomes and sophisticated gene expression networks. Consequently, chromatin regulators support this increased complexity. The ATP-dependent remodelers the SWI/SNF family are multiprotein complexes that modulate nucleosome positioning appear under different configurations, which perform distinct functions. While composition, architecture, activity these subclasses well understood in a limited number fungal animal model organisms, lack comprehensive information other eukaryotic precludes identification reliable evolutionary complexes. Here, we performed systematic analysis using 36 species animal, fungal, plant lineages assess conservation known subunits across eukaryotes. We identified relationships allowed us propose composition hypothetical ancestral last common ancestor. This ancestor appears have undergone several rounds lineage-specific subunit gains losses, shaping current conformation animals fungi. In addition, our results unravel complex, reminiscent BAF subclass, incorporates set plant-specific still unknown function.