Diversity of post-translational modifications and cell signaling revealed by single cell and single organelle mass spectrometry

• DOI: 10.1038/s42003-024-06579-7
• Publication Date: 2024-07-19T23:54:43Z
• Authors: Dong-Gi Mun; Firdous A. Bhat; Neha Joshi; Leticia Sandoval; Husheng Ding; Anu Jain; Jane A. Peterson; Taewook Kang; Ganesh P. Pujari; Jennifer L. Tomli...; Rohit Budhraja; Roman M. Zenka; Nagarajan Kannan; Benjamin R. Kipp; Surendra Dasari; Alexandre Gaspar-...; Rory L. Smoot; Richard K. Kandasamy; Akhilesh Pandey
• Keywords: Proteome; Single-Cell Analysis; Organelle; Cell Signaling

Abstract The rapid evolution of mass spectrometry-based single-cell proteomics now enables the cataloging several thousand proteins from single cells. We investigated whether we could discover cellular heterogeneity beyond proteome, encompassing post-translational modifications (PTM), protein-protein interaction, and variants. By optimizing spectrometry data interpretation strategy to enable detection PTMs variants, have generated a high-definition dataset nuclear proteomic-states. demonstrate cell-states signaling dependencies at level reveal epigenetic drug-induced changes in nuclei. This approach exploration previously uncharted organellar proteomes revealing molecular characteristics that are inaccessible through RNA profiling.