EV DNA from pancreatic cancer patient-derived cells harbors molecular, coding, non-coding signatures and mutational hotspots

• DOI: 10.1038/s42003-025-07567-1
• Publication Date: 2025-03-05T09:43:11Z
• Authors: Appolinaire A. Olou; Wesley A. Tom; Gary Krzyzanowski; Chao Jiang; Dinesh S. Chandel; Nirmalee Fernando; Adrian W. Draney; Joel Destino; Danny R. Welch; M. Rohan Fernando
• Keywords: Coding region

DNA packaged into cancer cell-derived EV is not well appreciated. Here, we uncovered signatures of secreted by pancreatic cells. The cells and non-cancer counterparts exhibit distinct low vs. high molecular weight (LMW HMW) fragments distribution, respectively. Genome sequencing Single Nucleotide Variants analysis revealed that 95% reads 94% SNVs map to noncoding regions the genome. Given ~1% human genome represents coding regions, 5% mapping rate suggests a non-random enrichment certain mutations. LMW only set apart, but also harbor specific unique top nine being FAM135B, COL22A1, TSNARE1, KCNK9, ZFAT, JRK, MROH5, GSDMD, MIR3667HG. Additionally, cells' dense centromeric more strikingly on chromosomes 3, 7, 9, 10, 11, 13, 17, 20. Mutational profiling turned up close 200 mutations for Altogether, our analyses suggest might hold clues content from cancer, molecular, mutational thereof, rationalizes need new approach biomarker research.