Double and single stranded detection of 5-methylcytosine and 5-hydroxymethylcytosine with nanopore sequencing

• DOI: 10.1038/s42003-025-07681-0
• Publication Date: 2025-02-15T17:10:26Z
• Authors: Dominic Oliver Ha...; Floris Honig; Stefan Bagby; Sandipan Roy; Adele Murrell
• Keywords: 5-Hydroxymethylcytosine; 5-Methylcytosine; Nanopore; Double stranded

5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) are modified versions of cytosine in DNA with roles regulating gene expression. Using whole genomic from mouse cerebellum, we benchmark 5mC 5hmC detection by Oxford Nanopore Technologies sequencing against other standard techniques. In addition, assess the ability duplex base-calling to study strand asymmetric modification. is accurate relative compared techniques opens means studying these modifications. Strand modification widespread across genome but reduced at imprinting control regions CTCF binding sites cerebellum. Here demonstrate unique nanopore improve resolution detail mapping.