TAT-CRE inhalation enables tumor induction corresponding to adenoviral Cre-recombinase in a lung cancer mouse model

• DOI: 10.1038/s42003-025-08146-0
• Publication Date: 2025-05-13T14:49:26Z
• Authors: Tabea Gewalt; Anna M. Dmitrieva; Felix Elsner; Xinlei Zhao; Daniel Dimitri Si...; Ilayda Gülsen Kocak; Qian Yang; Claudia Viktoria ...; Naja Maria Eckert; Bianca Goebel; Marieke Nill; Franziska Peter; Arndt Hartmann; Filippo Beleggia; Margarete Odenthal; Hans Christian Re...; Roland Tillmann U...; Frederik Graw; Lydia Meder
• Keywords: Cre recombinase

Abstract Cre-recombinase inducible model systems are extensively used in cancer research to manipulate gene expression specific tissues and induce autochthonous tumor growth. These often involve the cross-breeding of genetically engineered organisms containing loxP -flanked alleles with those expressing Cre-recombinase. This approach, while effective, has challenge requiring high numbers animals due breeding requirements. Other frequently induction methods direct application viral vectors. approach presents accessibility facilities that meet necessary safety level. In this context, we perform a comprehensive comparison between TAT-CRE (biosafety level S1) adenoviral induced S2) lung adenocarcinomas driven by Kras G12D Trp53 depletion. We use vivo monitoring via computed tomography, single-cell RNA sequencing, immunohistochemistry flow cytometry elucidate similarities differences adenocarcinomas. tumors present micro-vessels macrophages but corresponding onset growth characteristics compared adenoviral-Cre recombinase tumors. Taken together, is valuable genetic engineering S1 alternative for may be implemented other models than cancer.