Directly imaging emergence of phase separation in peroxidized lipid membranes

• DOI: 10.1038/s42004-022-00809-x
• Publication Date: 2023-01-17T10:56:20Z
• Authors: Miguel Paez-Perez; Aurimas Vyšniauskas; Ismael López-Duarte; Eulalie J. Lafarge; Raquel López-Ríos...; Carlos M. Marques; André P. Schroder; Pierre Muller; Christian D. Lorenz; Nicholas J. Brooks; Marina K. Kuimova
• Keywords: Separation (statistics)

Abstract Lipid peroxidation is a process which key in cell signaling and disease, it exploited cancer therapy the form of photodynamic therapy. The appearance hydrophilic moieties within bilayer’s hydrocarbon core will dramatically alter structure mechanical behavior membranes. Here, we combine viscosity sensitive fluorophores, advanced microscopy, X-ray diffraction molecular simulations to directly quantitatively measure structural viscoelastic properties, correlate these with atomistic modelling. Our results indicate an increase microviscosity decrease bending rigidity upon membranes, contrary trend observed non-oxidized lipids. Fluorescence lifetime imaging microscopy MD give evidence for presence membrane regions different local order oxidized We hypothesize that oxidation promotes stronger lipid-lipid interactions, lead lateral heterogeneity bilayer creation lipid clusters higher order.