Metabolic reprogramming of interleukin-17-producing γδ T cells promotes ACC1-mediated de novo lipogenesis under psoriatic conditions

• DOI: 10.1038/s42255-025-01276-z
• Publication Date: 2025-05-13T09:02:55Z
• Authors: Yu-San Kao; Mario Lauterbach; Aleksandra Lopez ...; Ute Distler; Gloria Janet Godoy; Matthias Klein; Rafael Jose Argüello; Fatima Boukhallouk; Sara Vallejo Fuente; Kathrin Luise Bra...; Assel Nurbekova; Monica Romero; Panagiota Mamareli; Luana Silva; Luis Eduardo Alve...; Francesca Rampoldi; Luciana Berod; Lydia Lynch; Karsten Hiller; Tim Sparwasser
• Keywords: Lipogenesis

Abstract Metabolic reprogramming determines γδ T cell fate during thymic development; however, the metabolic requirements of interleukin (IL)-17A-producing γδ T cells (γδT17 cells) under psoriatic conditions are unclear. Combining high-throughput techniques, including RNA sequencing, SCENITH, proteomics and stable isotope tracing, we demonstrated that psoriatic inflammation caused γδT17 cells to switch toward aerobic glycolysis. Under psoriatic conditions, γδT17 cells upregulated ATP-citrate synthase to convert citrate to acetyl-CoA, linking carbohydrate metabolism and fatty acid synthesis (FAS). Accordingly, we used a pharmacological inhibitor, Soraphen A, which blocks acetyl-CoA carboxylase (ACC), to impair FAS in γδT17 cells, reducing their intracellular lipid stores and ability to produce IL-17A under psoriatic conditions in vitro. We pinpointed the pathogenic role of ACC1 in γδT17 cells in vivo by genetic ablation, ameliorating inflammation in a psoriatic mouse model. Furthermore, ACC inhibition limited human IL-17A-producing γδT17 cells. Targeting ACC1 to attenuate pathogenic γδT17 cell function has important implications for psoriasis management.