Viral to metazoan marine plankton nucleotide sequences from the Tara Oceans expedition

• DOI: 10.1038/sdata.2017.93
• Publication Date: 2017-08-01T13:04:17Z
• Authors: Adriana Alberti; Julie Poulain; Stefan Engelen; Karine Labadie; Sarah Romac; Isabel Ferrera; Guillaume Albini; Jean-Marc Aury; Caroline Belser; Alexis Bertrand; Corinne Cruaud; Corinne Da Silva; Carole Dossat; Frédérick Gavory; Shahinaz Gas; Julie Guy; Maud Haquelle; E'krame Jacoby; Olivier Jaillon; Arnaud Lemainque; Eric Pelletier; Gaëlle Samson; Mark Wessner; ; Pascal Bazire; Odette Beluche; Laurie Bertrand; Marielle Besnard-...; Isabelle Bordelais; Magali Boutard; Maria Dubois; Corinne Dumont; Evelyne Ettedgui; Patricia Fernandez; Espérance Garcia; Nathalie Giordane...; Thomas Guerin; Chadia Hamon; Elodie Brun; Sandrine Lebled; Patricia Lenoble; Claudine Louesse; Eric Mahieu; Barbara Mairey; Nathalie Martins; Catherine Megret; Claire Milani; Jacqueline Muanga; Céline Orvain; Emilie Payen; Peggy Perroud; Emmanuelle Petit; Dominique Robert; Murielle Ronsin; Benoit Vacherie; Silvia G. Acinas; Marta Royo-Llonch; Francisco M. Corn...; Ramiro Logares; Beatriz Fernández...; Chris Bowler; Guy Cochrane; Clara Amid; Petra Ten Hoopen; Colomban De Vargas; Nigel Grimsley; Elodie Desgranges; Stefanie Kandels-...; Hiroyuki Ogata; Nicole Poulton; Michael E. Sieracki; Ramunas Stepanauskas; Matthew B. Sullivan; Jennifer R. Brum; Melissa B. Duhaime; Bonnie T. Poulos; Bonnie L. Hurwitz; ; Silvia G. Acinas; Peer Bork; Emmanuel Boss; Chris Bowler; Colomban De Vargas; Michael Follows; Gabriel Gorsky; Nigel Grimsley; Pascal Hingamp; Daniele Iudicone; Olivier Jaillon; Stefanie Kandels-...; Lee Karp-Boss; Eric Karsenti; Fabrice Not; Hiroyuki Ogata; Stéphane Pesant; Jeroen Raes; Christian Sardet; Michael E. Sieracki; Sabrina Speich; Lars Stemmann; Matthew B. Sullivan; Shinichi Sunagawa; Patrick Wincker; Stéphane Pesant; Eric Karsenti; Patrick Wincker
• Keywords: Statistics and Probability; Molecular Biology/Genomics [q-bio.GN]; 570; Data Descriptor; 0303 health sciences; Nucleotides; Oceans and Seas; [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry; Genomics; Library and Information Sciences; Plankton; Computer Science Applications; Education; 03 medical and health sciences; Cardiovascular and Metabolic Diseases; 13. Climate action; [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN]; Viruses; 14. Life underwater; Statistics, Probability and Uncertainty; Ecosystem; Information Systems

AbstractA unique collection of oceanic samples was gathered by the Tara Oceans expeditions (2009–2013), targeting plankton organisms ranging from viruses to metazoans, and providing rich environmental context measurements. Thanks to recent advances in the field of genomics, extensive sequencing has been performed for a deep genomic analysis of this huge collection of samples. A strategy based on different approaches, such as metabarcoding, metagenomics, single-cell genomics and metatranscriptomics, has been chosen for analysis of size-fractionated plankton communities. Here, we provide detailed procedures applied for genomic data generation, from nucleic acids extraction to sequence production, and we describe registries of genomics datasets available at the European Nucleotide Archive (ENA, www.ebi.ac.uk/ena). The association of these metadata to the experimental procedures applied for their generation will help the scientific community to access these data and facilitate their analysis. This paper complements other efforts to provide a full description of experiments and open science resources generated from the Tara Oceans project, further extending their value for the study of the world’s planktonic ecosystems.