A tissue-based draft map of the murine MHC class I immunopeptidome

• DOI: 10.1038/sdata.2018.157
• Publication Date: 2018-08-07T13:10:20Z
• Authors: Heiko Schuster; Wenguang Shao; Tobias Weiss; Patrick G.A. Pedr...; Patrick Roth; Michael Weller; David S. Campbell; Eric W. Deutsch; Robert L. Moritz; Oliver Planz; Hans-Georg Rammensee; Ruedi Aebersold; Etienne Caron
• Keywords: Statistics and Probability; 0301 basic medicine; Data Descriptor; 610 Medicine & health; Library and Information Sciences; 1710 Information Systems; 7. Clean energy; Mass Spectrometry; Education; Mice; 03 medical and health sciences; 1706 Computer Science Applications; Animals; 1804 Statistics, Probability and Uncertainty; 2613 Statistics and Probability; 0303 health sciences; Histocompatibility Antigens Class I; 10040 Clinic for Neurology; Computer Science Applications; 3. Good health; Mice, Inbred C57BL; Organ Specificity; 3309 Library and Information Sciences; Statistics, Probability and Uncertainty; Peptides; 3304 Education; Information Systems

Abstract The large array of peptides presented to CD8+ T cells by major histocompatibility complex (MHC) class I molecules is referred as the MHC immunopeptidome. Although immunopeptidome ubiquitous in mammals and represents a critical component immune system, very little known, any species, about its composition across most tissues organs vivo . We applied mass spectrometry (MS) technologies draft first tissue-based atlas murine health. Peptides were extracted from 19 normal C57BL/6 mice prepared for MS injections, resulting total number 28,448 high-confidence H2D b /K -associated identified annotated atlas. This provides initial qualitative data explore tissue-specificity serves guide identify potential tumor-associated antigens various cancer models. Our shared via PRIDE (PXD008733), SysteMHC Atlas (SYSMHC00018) SWATH Atlas. anticipate that this unique dataset will be expanded future find wide applications basic translational immunology.