Identification of retinoic acid-regulated nuclear matrix-associated protein as a novel regulator of gastric cancer

• DOI: 10.1038/sj.bjc.6605202
• Publication Date: 2009-08-11T22:52:26Z
• Authors: J Li; E K O Ng; Y P Ng; C Y P Wong; J Yu; H Jin; V Y Y Cheng; M Y Y Go; P K F Cheung; M P A Ebert; J Tong; K F To; F K L Chan; J J Y Sung; N Y Ip; W K Leung
• Keywords: Viability assay

Background: Retinoic acid-regulated nuclear matrix-associated protein (RAMP) is a WD40 repeat-containing that involved in various biological functions, but little known about its role human cancer. This study aims to delineate the oncogenic of RAMP gastric carcinogenesis. Methods: expression was examined by real-time quantitative RT-PCR, immunohistochemistry and western blotting. Inhibition performed siRNA-mediated knockdown. The functional effects on cell kinetics were measured viability assay, colony formation assay flow cytometry. Cell lines stably expressing established investigate vitro. Results: Ramp readily expressed all seven cancer significantly increased tissues when compared with their adjacent non-cancerous (P<0.001). In keeping this, higher tissues, whereas moderate noted intestinal metaplasia. Knockdown cells reduced proliferation (P<0.01) soft agar (P<0.001), induced apoptosis G2/M arrest. additional, knockdown dependent accumulation p53 p21 induction cleaved caspases-9, caspases-3 PARP. Strikingly, overexpression promoted anchorage-independent growth agar. Conclusion: Our findings demonstrate plays an may be promising approach for therapy.