Effect of dietary phenolic compounds on apoptosis of human cultured endothelial cells induced by oxidized LDL

• DOI: 10.1038/sj.bjp.0701624
• Publication Date: 2005-01-28T19:19:31Z
• Authors: Otilia Vieira; Isabelle Escargue...; Olivier Meilhac; Jean‐Pierre Basile; Joao Laranjinha; Leonor Almeida; Robert Salvayre; Anne Nègre‐Salvayre
• Keywords: TBARS; Fragmentation; Ellagic Acid

Oxidized low density lipoproteins (LDL) are toxic to cultured endothelial cells. Mildly oxidized LDL, characterized by relatively levels of TBARS and only minor modifications apoB, were obtained using 2 experimental model systems oxidation, namely oxidation u.v. radiation or ferrylmyoglobin (a two electron product from the reaction metmyoglobin with H O ). Toxic concentrations mildly LDL induce apoptosis (programmed cell death) cells, as shown typical morphological features, in situ TUNEL procedure DNA fragmentation revealed on gel electrophoresis. This is calcium‐dependent subsequent intense sustained cytosolic [Ca 2+ ] i peak elicited LDL. Five naturally occurring phenolic compounds present food beverages able prevent, a concentration‐dependent manner, cells induced Among tested, caffeic acid was most effective. Under conditions used, protective effect (IC 50 8.3±2.1 μmol l −1 ) prevention significantly higher than that other tested s 12.4±3.2, 14.1±4.1, 20.4±4.4 72.6±9.2 for ferulic, protocatechuic, ellagic p ‐coumaric acids, respectively). The anti‐apoptotic results addition effects, (i) antioxidant which prevents toxicity (‘indirect’ effect); (ii) ‘direct’ cytoprotective effect, acting at cellular level. Effective acted level blocking rise In conclusion, acids (caffeic ferulic being potent under used) exhibit against delaying acts agent, probably intracellular signalling triggered culminating calcium involved LDL‐induced apoptosis. British Journal Pharmacology (1998) 123 , 565–573; doi: 10.1038/sj.bjp.0701624