DNA mediated chromatin pull-down for the study of chromatin replication

• DOI: 10.1038/srep00095
• Publication Date: 2011-09-19T16:02:34Z
• Authors: Anna E. Kliszczak; Michael D. Rainey; Brendan Harhen; Francois M. Boisvert; Corrado Santocanale
• Keywords: Origin recognition complex; ChIA-PET

Chromatin replication involves duplicating DNA while maintaining epigenetic information. These processes are critical for genome stability and preserving cell-type identity. Here we describe a simple experimental approach that allows chromatin to be captured its content analysed after in vivo labeling of by cellular polymerases. We show this technique is highly specific proteins bound the replicated can analyzed both immunological techniques large scale mass spectrometry. As proof concept have used novel procedure begin investigating relationship between protein composition temporal programme human cells. It expected will become widely tool address how assemble onto newly passage fork maturation coupled synthesis.