A physical sciences network characterization of non-tumorigenic and metastatic cells

• DOI: 10.1038/srep01449
• Publication Date: 2013-04-26T09:05:36Z
• Authors: David B. Agus; Jenolyn F. Alexander; Wadih Arap; Shashanka Ashili; Joseph E. Aslan; Robert H. Austin; Vadim Backman; Kelly J. Bethel; Richard Bonneau; Wei-Chiang Chen; Chira Chen-Tanyolac; Nathan C. Choi; Steven A. Curley; Matthew Dallas; Dhwanil Damania; Paul C. W. Davies; Paolo Decuzzi; Laura Dickinson; Luis Estevez-Salm...; Veronica Estrella; Mauro Ferrari; Claudia Fischbach; Jasmine Foo; Stephanie I. Fraley; Christian Frantz; Alexander Fuhrmann; Philippe Gascard; Robert A. Gatenby; Yue Geng; Sharon Gerecht; Robert J. Gillies; Biana Godin; William M. Grady; Alex Greenfield; Courtney Hemphill; Barbara L. Hempstead; Abigail Hielscher; W. Daniel Hillis; Eric C. Holland; Arig Ibrahim-Hashim; Tyler Jacks; Roger H. Johnson; Ahyoung Joo; Jonathan E. Katz; Laimonas Kelbauskas; Carl Kesselman; Michael R. King; Konstantinos Kons...; Casey M. Kraning-...; Peter Kuhn; Kevin Kung; Brian Kwee; Johnathon N. Lakins; Guillaume Lambert; David Liao; Jonathan D. Licht; Jan T. Liphardt; Liyu Liu; Mark C. Lloyd; Anna Lyubimova; Parag Mallick; John Marko; Owen J. T. McCarty; Deirdre R. Meldrum; Franziska Michor; Shannon M. Mument...; Vivek Nandakumar; Thomas V. O’Halloran; Steve Oh; Renata Pasqualini; Matthew J. Paszek; Kevin G. Philips; Christopher S. Po...; Kuldeepsinh Rana; Cynthia A. Reinha...; Robert Ros; Gregg L. Semenza; Patti Senechal; Michael L. Shuler; Srimeenakshi Srin...; Jack R. Staunton; Yolanda Stypula; Hariharan Subrama...; Thea D. Tlsty; Garth W. Tormoen; Yiider Tseng; Alexander van Oud...; Scott S. Verbridge; Jenny C. Wan; Valerie M. Weaver; Jonathan Widom; Christine Will; Denis Wirtz; Jonathan Wojtkowiak; Pei-Hsun Wu
• Keywords: Cancer cell lines

To investigate the transition from non-cancerous to metastatic a physical sciences perspective, Physical Sciences-Oncology Centers (PS-OC) Network performed molecular and biophysical comparative studies of non-tumorigenic MCF-10A MDA-MB-231 breast epithelial cell lines, commonly used as models cancer metastasis. Experiments were in 20 laboratories 12 PS-OCs. Each laboratory was supplied with identical aliquots common reagents culture protocols. Analyses these measurements revealed dramatic differences their mechanics, migration, adhesion, oxygen response, proteomic profiles. Model-based multi-omics approaches identified key between cells' regulatory networks involved morphology survival. These results provide multifaceted description cellular parameters two widely lines demonstrate value PS-OC approach for integration diverse experimental observations elucidate phenotypes associated