Long-term strain improvements accumulate mutations in regulatory elements responsible for hyper-production of cellulolytic enzymes

• DOI: 10.1038/srep01569
• Publication Date: 2013-03-28T10:10:16Z
• Authors: Guodong Liu; Lei Zhang; Yuqi Qin; Gen Zou; Zhonghai Li; Xing Yan; Xiaomin Wei; Mei Chen; Ling Chen; Kai Zheng; Jun Zhang; Liang Ma; Jie Li; Rui Liu; Hai Xu; Xiaoming Bao; Xu Fang; Lushan Wang; Yaohua Zhong; Weifeng Liu; Huajun Zheng; Shengyue Wang; Chengshu Wang; Luying Xun; Guo-Ping Zhao; Tianhong Wang; Zhihua Zhou; Yinbo Qu
• Keywords: Strain (injury); Wild type

Long-term strain improvements through repeated mutagenesis and screening have generated a hyper-producer of cellulases hemicellulases from Penicillium decumbens 114 which was isolated 30 years ago. Here, the genome P. JU-A10-T sequenced compared with that wild-type 114-2. Further, transcriptomes secretomes were between strains. Selective hyper-production but not all secreted proteins observed in mutant, making it more specific producer lignocellulolytic enzymes. Functional analysis identified changes several transcriptional regulatory elements played crucial roles cellulase hyper-producing characteristics mutant. Additionally, mutant showed enhanced supply amino acids decreased synthesis secondary metabolites wild-type. The results clearly point out we can target gene regulators promoters minimal alterations genetic content maximal effects engineering.