Targeting microRNA-23a to inhibit glioma cell invasion via HOXD10

• DOI: 10.1038/srep03423
• Publication Date: 2013-12-05T10:10:34Z
• Authors: Xing Hu; Dan Chen; Yanhui Cui; Zhiyuan Li; Jufang Huang
• Keywords: Homeodomain Proteins; 0301 basic medicine; Brain Neoplasms; Glioma; Article; Gene Expression Regulation, Neoplastic; MicroRNAs; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Humans; RNA Interference; RNA, Messenger; Transcription Factors

AbstractGlioma is the most frequent primary brain tumor. Recently, the upregulation of microRNA (miR)-23a was found to be associated with glioma, but the molecular mechanism by which miR-23a promotes glioma growth remains to be unveiled. In the present study, we found that miR-23a was significantly upregulated in glioma tissues compared to their matched adjacent tissues. miR-23a was also highly expressed in glioma cell lines SHG44, U251 and U87 cells. Moreover, we identified homeobox D10 (HOXD10) as a novel target for miR-23a. The expression of HOXD10 was significantly reduced in glioma tissues and cell lines and miR-23a negatively regulates the protein expression of HOXD10 in U251 and U87 cells. We further showed that miRNA-23a promoted U251 and U87 cell invasion, at least partially, by directly targeting HOXD10 and further modulating MMP-14. These findings suggest that miR-23a may serve as a promising therapeutic target for glioma.