Eriocitrin ameliorates diet-induced hepatic steatosis with activation of mitochondrial biogenesis

• DOI: 10.1038/srep03708
• Publication Date: 2014-01-15T10:13:28Z
• Authors: Masanori Hiramitsu; Yasuhito Shimada; Junya Kuroyanagi; Takashi Inoue; Takao Katagiri; Liqing Zang; Yuhei Nishimura; Norihiro Nishimura; Toshio Tanaka
• Keywords: Steatosis; NRF1; TFAM; Organelle biogenesis

Lemon (Citrus limon) contains various bioactive flavonoids and prevents obesity obesity-associated metabolic diseases. We focused on eriocitrin (eriodictyol 7-rutinoside), a powerful antioxidative flavonoid in lemon with lipid-lowering effects rat model of high-fat diet. To investigate the mechanism action eriocitrin, we conducted feeding experiments zebrafish diet-induced obesity. Oral administration (32 mg/kg/day for 28 days) improved dyslipidaemia decreased lipid droplets liver. DNA microarray analysis revealed that increased mRNA mitochondrial biogenesis genes, such as mitochondria transcription factor, nuclear respiratory factor 1, cytochrome c oxidase subunit 4 ATP synthase. In HepG2 cells, also induced corresponding orthologues reduced accumulation under conditions loading. Eriocitrin size mtDNA content, which resulted production cells zebrafish. summary, dietary ameliorates hepatic steatosis activation biogenesis.