Species identification through mitochondrial rRNA genetic analysis

• DOI: 10.1038/srep04089
• Publication Date: 2014-02-13T10:13:51Z
• Authors: Li Yang; Zongqing Tan; Daren Wang; Ling Xue; Min-xin Guan; Taosheng Huang; Ronghua Li
• Keywords: Ribosomal; 16S; 0303 health sciences; Base Sequence; Sequence Analysis, RNA; Molecular Sequence Data; Polymerase Chain Reaction; Article; Mitochondria; Databases; 03 medical and health sciences; Genetic; RNA, Ribosomal; RNA, Ribosomal, 16S; Databases, Genetic; Genetics; RNA; Animals; Humans; Generic health relevance; Sequence Analysis; Sequence Alignment

Inter-species and intraspecific variations in mitochondrial DNA (mtDNA) were observed in a bioinformatics analysis of the mitochondrial genomic sequences of 11 animal species. Some highly conserved regions were identified in the mitochondrial 12S and 16S ribosomal RNA (rRNA) genes of these species. To test whether these sequences are universally conserved, primers were designed to target the conserved regions of these two genes and were used to amplify DNA from 21 animal tissues, including two of unknown origin. By sequencing these PCR amplicons and aligning the sequences to a database of non-redundant nucleotide sequences, it was confirmed that these amplicons aligned specifically to mtDNA sequences from the expected species of origin. This molecular technique, when combined with bioinformatics, provides a reliable method for the taxonomic classification of animal tissues.