Usefulness of circulating microRNAs for the prediction of early preeclampsia at first-trimester of pregnancy

• DOI: 10.1038/srep04882
• Publication Date: 2014-05-08T14:13:05Z
• Authors: Ana Luque; Abduljalil Farwati; Francesca Crovetto; Fatima Crispi; Francesc Figueras; Eduard Gratacós; Josep M. Aran
• Keywords: Adult; 0301 basic medicine; Micro RNAs; Article; 3. Good health; Hypertension in pregnancy; MicroRNAs; Pregnancy Trimester, First; 03 medical and health sciences; Pre-Eclampsia; Pregnancy; Case-Control Studies; Humans; Female; Hipertensió en l'embaràs; Biomarkers

To assess the usefulness of circulating microRNAs (miRNAs) as non-invasive molecular biomarkers for early prediction preeclampsia, a differential miRNA profiling analysis was performed in first-trimester pooled sera from 31 preeclampsia patients, requiring delivery before 34 weeks gestation and 44 uncomplicated pregnancies using microfluidic arrays. Among total 754 miRNAs analyzed, presence 63 (8%) consistently documented control samples. Nevertheless, only 15 amplified (2%) seemed to be differentially, although modestly, represented (fold change range: 0.4–1.4). After stem loop RT-qPCR individual samples, statistical confirmed that none most consistent differentially (3 overrepresented 4 underrepresented) were abundant serum preeclamptic compared with normal pregnancies. Therefore, maternal assessment at pregnancy does not appear have any predictive value preeclampsia.