Label-free cell phenotypic profiling decodes the composition and signaling of an endogenous ATP-sensitive potassium channel

• DOI: 10.1038/srep04934
• Publication Date: 2014-05-12T09:06:57Z
• Authors: Haiyan Sun; Ying Wei; Huayun Deng; Qiaojie Xiong; Min Li; Joydeep Lahiri; Ye Fang
• Keywords: Pinacidil; ATP-sensitive potassium channel

Abstract Current technologies for studying ion channels are fundamentally limited because of their inability to functionally link channel activity cellular pathways. Herein, we report the use label-free cell phenotypic profiling decode composition and signaling an endogenous ATP-sensitive potassium (K ATP ) in HepG2C3A, a hepatocellular carcinoma line. Label-free agonist showed that pinacidil triggered characteristically similar dynamic mass redistribution (DMR) signals A431, A549, HT29 but not HepG2 cells. Reverse transcriptase PCR, RNAi knockdown K blocker DMR is due activation SUR2/Kir6.2 HepG2C3A Kinase inhibition activated trigger through Rho kinase Janus kinase-3 cause actin remodeling. The results first demonstration methodology characterize channel.