Detecting protein–protein interactions based on kinase-mediated growth induction of mammalian cells
0301 basic medicine
0303 health sciences
Proto-Oncogene Proteins c-mdm2
Article
Tacrolimus
6. Clean water
Cell Line
3. Good health
Tacrolimus Binding Proteins
Mice
Proto-Oncogene Proteins c-kit
03 medical and health sciences
Amino Acid Substitution
Protein Interaction Mapping
Animals
Protein Interaction Domains and Motifs
Tumor Suppressor Protein p53
Peptides
Dimerization
Cell Proliferation
DOI:
10.1038/srep06127
Publication Date:
2014-08-19T09:04:48Z
AUTHORS (3)
ABSTRACT
Detection of protein–protein interactions (PPIs) is important for understanding numerous processes in mammalian cells; however, existing PPI detection methods often give significant background signals. Here, we propose a novel PPI-detection method based on kinase-mediated growth induction cells. In this method, target proteins are fused to the intracellular domain c-kit (c-kit ICD) and expressed interleukin-3-dependent The induces dimerization activation ICDs, which leads cell absence interleukin-3. Using system, successfully detected ligand-dependent homo-interaction FKBPF36V hetero-interaction FKBP FRBT2098L, as well constitutive interaction between MDM2 known peptide inhibitor. Intriguingly, cells expressing high-affinity chimeras selected from mixture populations dominantly low-affinity chimeras. These results indicate that can detect PPIs with low levels suitable inhibitor screening.
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CITATIONS (23)
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