Integrated miRNA and mRNA expression profiling to identify mRNA targets of dysregulated miRNAs in non-obstructive azoospermia

• DOI: 10.1038/srep07922
• Publication Date: 2015-01-28T10:27:21Z
• Authors: Xuan Zhuang; Zhiming Li; Huinuan Lin; Long Gu; Qing Lin; Zhongxian Lu; Chi-Meng Tzeng
• Keywords: Obstructive azoospermia

The aim of this study was to identify mRNA targets dysregulated miRNAs through the integrated analysis miRNA and expression profiling in men with normal versus impaired spermatogenesis. mRNAs testicular tissues obtained from males non-obstructive azoospermia (NOA, n = 4) or obstructive (OA, 3) spermatogenesis analyzed using microarray technology. Some most interesting results were validated by real time PCR samples same cohort. Ninety-three 4172 differentially expressed NOA normozoospermic OA patients. In addition confirming that significantly genes play pivotal roles NOA, promising correlation signatures these miRNA/mRNA pairs discovered study. functional classification revealed frequently associated spermatogenesis, cell meiosis, cycle development secondary male sexual characteristics. This is first systematic both patients OA. Our indicate phenotypic status characterized dysfunction when compared males.