Neuronal exosomes facilitate synaptic pruning by up-regulating complement factors in microglia

• DOI: 10.1038/srep07989
• Publication Date: 2015-01-23T10:14:32Z
• Authors: Insaf Bahrini; Ji-hoon Song; Diego Diez; Rikinari Hanayama
• Keywords: Neurite; Synaptic Pruning; Exosome

Selective elimination of synaptic connections is a common phenomenon which occurs during both developmental and pathological conditions. Glial cells have central role in the pruning synapses by specifically engulfing degenerating neurites inappropriate connections, but its regulatory mechanisms been largely unknown. To identify mediators this process, we established an vitro cell culture assay for synapse elimination. Neuronal differentiation formation PC12 were induced culturing with nerve growth factor (NGF) serum-free medium. trigger elimination, NGF-containing medium was replaced DMEM containing 10% FBS degenerated within two days. Co-culturing MG6 cells, mouse microglial line, accelerated removal phagocytosis. When pre-incubated exosomes secreted from differentiated after depolarization, further increasing expression levels complement component 3 cells. These results define as regulator clarify novel mechanism whereby active promote inactive ones stimulating phagocytosis exosomes.