Apoptosis Signal-regulating Kinase 1 promotes Ochratoxin A-induced renal cytotoxicity

• DOI: 10.1038/srep08078
• Publication Date: 2015-01-28T10:06:57Z
• Authors: Rui Liang; Xiao Li Shen; Boyang Zhang; Yuzhe Li; Wentao Xu; Changhui Zhao; YunBo Luo; Kunlun Huang
• Keywords: ASK1; Kinome; Cyclin-dependent kinase 9

Abstract Oxidative stress and apoptosis are involved in Ochratoxin A (OTA)-induced renal cytotoxicity. Apoptosis signal-regulating kinase 1 (ASK1) is a Mitogen-Activated Protein Kinase (MAPKKK, MAP3K) family member that plays an important role oxidative stress-induced cell apoptosis. In this study, we performed RNA interference of ASK1 HEK293 cells employed iTRAQ-based quantitative proteomics approach to globally investigate the regulatory mechanism OTA-induced Our results showed knockdown alleviated ROS generation Δψm loss thus desensitized We identified 33 24 differentially expressed proteins upon OTA treatment scrambled cells, respectively. Pathway classification analysis revealed participated inhibition mRNA splicing, nucleotide metabolism, cycle, DNA repair activation lipid metabolism. concluded essential promoting