Glycerol-3-phosphate O-acyltransferase is required for PBAN-induced sex pheromone biosynthesis in Bombyx mori

• DOI: 10.1038/srep08110
• Publication Date: 2015-01-29T12:20:59Z
• Authors: Mengfang Du; Xiaoguang Liu; Xiaoming Liu; Xinming Yin; Shuangyin Han; Qisheng Song; Shiheng An
• Keywords: Acyltransferases; Acyltransferases

Abstract Female moths employ their own pheromone blends as a communicational medium in mating behavior. The biosynthesis and release of sex female are regulated by activating neuropeptide (PBAN) the corresponding action PBAN has been well elucidated Bombyx mori. However, very little is known about molecular mechanism regarding precursor. In this study, quantitative proteomics was utilized to comprehensively elucidate expression dynamics glands (PGs) during development. Proteomic analysis revealed serial differentially expressed biosynthesis-associated proteins at different time points B. mori Most interestingly glycerol-3-phosphate O-acyltransferase (BmGPAT) found be key periods biosynthesis. RNAi knockdown BmGPAT confirmed important function protein precursor, triacylglcerol (TAG) subsequently PBAN-induced production pheromone, bombykol. Behavioral showed that GPAT significantly impaired ability females attract males. Our findings indicate acts regulate TAG, thus influencing subsequent