Annexin A2 as a target endothelial cell membrane autoantigen in Behçet's disease

• DOI: 10.1038/srep08162
• Publication Date: 2015-02-02T10:03:04Z
• Authors: Peng Chen; Hai Yan; Yaping Tian; Yiping Xun; Lili Shi; Ran Bao; Huai Zhang; Guangyu Chen; Chunhe Yang; Shutao Sun; Yajie Wang; Li Liu; Yabin Zhou; Chunyan Zhang; Xiaoxu Wang; Yongqiang Wen; Yongzhong Bian; Hongwu Du
• Keywords: Annexin A2; Annexin A1; Pathogenesis; Annexin A5

Cell membrane proteins are believed to play a critical role in the pathogenesis of autoimmune diseases. However, few autoantigens have been linked with Behçet's disease. Here, cell-chip was performed identify autoantibody target cells, and suspected were detected using immunoblotting. The amino acid sequences determined LC-MALDI-TOF/TOF. Putative recombinantly expressed purified, corresponding ELISA developed clinically validated real clinical samples. It found that 36-kDa protein--annexin A2--was approximately one-third patients' blood circulation. immunohistochemistry results showed annexin A2 highly vascular endothelial cells. Moreover, involvement significantly higher anti-annexin antibody-positive group versus antibody-negative among all samples analyzed, indicating is novel cell antigen involved