Protein phosphorylation detection using dual-mode field-effect devices and nanoplasmonic sensors

• DOI: 10.1038/srep08687
• Publication Date: 2015-03-03T11:13:19Z
• Authors: Nikhil Bhalla; Mirella Di Lorenzo; Giordano Pula; Pedro Estrela
• Keywords: Dual mode; Protein detection

Abstract Phosphorylation by kinases is an important post-translational modification of proteins. It a critical control for the regulation vital cellular activities and its dysregulation implicated in several diseases. A common drug discovery approach involves, therefore, time-consuming screenings large libraries candidate compounds to identify novel inhibitors protein kinases. In this work, we propose method that combines localized surface plasmon resonance (LSPR) electrolyte insulator semiconductor (EIS)-based proton detection rapid identification kinase inhibitors. particular, selective thiophosphorylated proteins LSPR achieved changing their properties via pre-binding with gold nanoparticles. parallel, EIS field-effect structure allows real-time electrochemical monitoring phosphorylation detecting release protons associated activity. This innovative combination both nanoplasmonic sensing makes more reliable effective. As result, screening becomes rapid, sensitive, robust cost-effective.