Long-tip high-speed atomic force microscopy for nanometer-scale imaging in live cells

• DOI: 10.1038/srep08724
• Publication Date: 2015-03-04T10:19:24Z
• Authors: Mikihiro Shibata; Takayuki Uchihashi; Toshio Ando; Ryohei Yasuda
• Keywords: Nanometre; Filopodia; Live cell imaging; Microscale chemistry

Visualization of morphological dynamics live cells with nanometer resolution under physiological conditions is highly desired, but challenging. It has been demonstrated that high-speed atomic force microscopy a powerful technique for visualizing biomolecules conditions. However, application imaging larger objects such as mammalian complicated because the collision between cantilever and samples. Here, we demonstrate attaching an extremely long (~3 μm) thin (~5 nm) tip by amorphous carbon to allows us image surface structure spatiotemporal nanometers seconds. We long-tip capable morphogenesis filopodia, membrane ruffles, pit formation, endocytosis in COS-7, HeLa hippocampal neurons.