Calcium Spikes in Epithelium: study on Drosophila early embryos

• DOI: 10.1038/srep11379
• Publication Date: 2015-07-22T09:04:05Z
• Authors: Olga Markova; Sébastien Sénatore; Claire Chardès; Pierre-François L...
• Keywords: 0301 basic medicine; [SDV]Life Sciences [q-bio]; WAVES; TRANSIENTS; Action Potentials; [SDV.BC]Life Sciences [q-bio]/Cellular Biology; ORGANIZATION; Article; Epithelium; [SDV] Life Sciences [q-bio]; CA2+; 03 medical and health sciences; XENOPUS; CELLS; MORPHOGENESIS; Animals; Calcium; Drosophila; GASTRULATION; ZEBRAFISH DEVELOPMENT; FORCES; [SDV.BC] Life Sciences [q-bio]/Cellular Biology

AbstractCalcium ion acts in nearly every aspect of cellular life. The versatility and specificity required for such a ubiquitous role is ensured by the spatio-temporal dynamics of calcium concentration variations. While calcium signal dynamics has been extensively studied in cell cultures and adult tissues, little is known about calcium activity during early tissue morphogenesis. We monitored intracellular calcium concentration in Drosophila gastrula and revealed single cell calcium spikes that were short-lived, rare and showed strong variability among embryos. We quantitatively described the spatio-temporal dynamics of these spikes and analyzed their potential origins and nature by introducing physical and chemical perturbations. Our data highlight the inter- and intra-tissue variability of calcium activity during tissue morphogenesis.