Investigation of multipotent postnatal stem cells from human maxillary sinus membrane

• DOI: 10.1038/srep11660
• Publication Date: 2015-06-29T11:09:06Z
• Authors: JunBing Guo; JunQuan Weng; Qiong Rong; Xing Zhang; ShuangXi Zhu; DaiYing Huang; Xiang Li; Song Ling Chen
• Keywords: Adipogenesis; Adolescent; Periodontal Ligament; Multipotent Stem Cells; Bone Marrow Cells; Cell Separation; Maxillary Sinus; Immunohistochemistry; Article; Clone Cells; Immunophenotyping; Radiography; Adult Stem Cells; Young Adult; 03 medical and health sciences; 0302 clinical medicine; Osteogenesis; 13. Climate action; Humans; Chondrogenesis; Dental Pulp; Stem Cell Transplantation

Abstract Maxillary sinus membrane (MSM) elevation is a common surgical technique for increasing bone height in the posterior maxilla prior to dental implant placement. However, biological nature of regeneration MSM remains largely unidentified. In this study, tissue was obtained from 16 individuals during orthognathic surgery and used isolate stem cells (MSMSCs) by single-colony selection STRO-1 cell sorting. The characteristics terms colony-forming ability, surface antigens, multi-differentiation potential vivo implantation were all evaluated. It found that MSMSCs mesenchymal origin positive (MSC) markers such as STRO-1, CD146, CD29 CD44; furthermore, under defined culture conditions, able form mineral deposits differentiate into adipocytes chondrocytes. When transplanted immunocompromised rodents, showed capacity generate bone-like and, importantly, maintain their MSC after implantation. These findings provide cellular molecular evidence contains show functional implant.