Escherichia coli as host for membrane protein structure determination: a global analysis

• DOI: 10.1038/srep12097
• Publication Date: 2015-07-10T12:21:09Z
• Authors: Georges Hattab; Dror E. Warschawski; Karine Moncoq; Bruno Miroux
• Keywords: Inducer; T7 RNA polymerase

Abstract The structural biology of membrane proteins (MP) is hampered by the difficulty in producing and purifying them. A comprehensive analysis protein databases revealed that 213 unique structures have been obtained after production target E. coli . primary expression system used was one based on T7 RNA polymerase, followed arabinose T5 promoter systems. C41λ(DE3) C43λ(DE3) bacterial mutant hosts contributed to 28% non structures. large scale protocols demonstrated a preference for combination host-vector together with bimodal distribution induction temperature inducer concentration. Altogether our provides set rules optimal use systems production.