Computational Prediction and Validation of BAHD1 as a Novel Molecule for UlcerativeColitis

• DOI: 10.1038/srep12227
• Publication Date: 2015-07-17T09:40:12Z
• Authors: Huatuo Zhu; Xingyong Wan; Jing Li; Lu Han; Xiaochen Bo; Wenguo Chen; Chao Lu; Zhe Shen; Chenfu Xu; Lihua Chen; Chaohui Yu; Guoqiang Xu
• Keywords: 0303 health sciences; Chromosomal Proteins, Non-Histone; JNK Mitogen-Activated Protein Kinases; NF-kappa B; Gene Expression; Reproducibility of Results; Models, Biological; Article; Cell Line; Disease Models, Animal; Gene Knockout Techniques; Mice; 03 medical and health sciences; Receptors, Tumor Necrosis Factor, Type I; Animals; Humans; Colitis, Ulcerative; Computer Simulation; Genetic Predisposition to Disease; I-kappa B Proteins; Inflammation Mediators; RNA, Small Interfering; Signal Transduction

Abstract Ulcerative colitis (UC) is a common inflammatory bowel disease (IBD) producingintestinal inflammation and tissue damage. The precise aetiology of UC remainsunknown. In this study, we applied rank-based expression profile comparativealgorithm, gene set enrichment analysis (GSEA), to evaluate the profilesof patients small interfering RNA (siRNA)-perturbed cells predict proteinsthat might be essential in from publicly available profiles. We usedquantitative PCR (qPCR) characterize levels those genespredicted most important for dextran sodium sulphate (DSS)-inducedcolitic mice. found that bromo-adjacent homology domain (BAHD1), novelheterochromatinization factor vertebrates, was downregulated gene. Wefurther validated potential role BAHD1 as regulatory inflammationthrough TNF signalling pathway vitro . Our findings indicate thatcomputational approaches leveraging public data can used inferpotential genes or proteins diseases act an indispensablefactor regulating cellular response UC.