A Novel Intravital Imaging Window for Longitudinal Microscopy of the Mouse Ovary

• DOI: 10.1038/srep12446
• Publication Date: 2015-07-24T12:37:10Z
• Authors: Filip Bochner; Liat Fellus-Alyagor; Vyacheslav Kalchenko; Shiri Shinar; Michal Neeman
• Keywords: Intravital microscopy; Decellularization

Abstract The ovary is a dynamic organ that undergoes dramatic remodeling throughout the ovulatory cycle. Maturation of ovarian follicle, release oocyte in course ovulation as well formation and degradation corpus luteum involve tightly controlled extracellular matrix vasculature. Ovarian tumors, regardless their tissue origin, dynamically interact with microenvironment. Their activity encompasses recruitment host stroma immune cells, attachment tumor cells to mesothelial layer, cell migration. High-resolution imaging such processes particularly challenging for internal organs. implementation novel window reported here enabled longitudinal microscopy physiology orthotopic invasion.