Putting on the brakes: Bacterial impediment of wound healing

• DOI: 10.1038/srep14003
• Publication Date: 2015-09-14T09:29:14Z
• Authors: Kimberly M. Brothers; Nicholas A. Stella; Kristin M. Hunt; Eric G. Romanowski; Xinyu Liu; Jes K. Klarlund; Robert M. Q. Shanks
• Keywords: Ex vivo

The epithelium provides a crucial barrier to infection, and its integrity requires efficient wound healing. Bacterial cells secretomes from subset of tested species bacteria inhibited human porcine corneal epithelial cell migration in vitro ex vivo. Secretomes 95% Serratia marcescens, 71% Pseudomonas aeruginosa, 29% Staphylococcus aureus strains, other bacterial migration. Migration foreskin fibroblasts was also by S. marcescens indicating that the effect is not cornea specific. Transposon mutagenesis implicated lipopolysaccharide (LPS) core biosynthetic genes as being required inhibit LPS depletion with polymyxin B agarose rendered unable Purified but Escherichia coli or strains mutations waaG waaC genes, healing Together these data suggest sufficient for inhibition This study presents novel host-pathogen interaction implications infections where impact evidence secreted key factor inhibitory mechanism.