Identification of bolting-related microRNAs and their targets reveals complex miRNA-mediated flowering-time regulatory networks in radish (Raphanus sativus L.)
Bolting
Raphanus
DOI:
10.1038/srep14034
Publication Date:
2015-09-15T09:12:24Z
AUTHORS (10)
ABSTRACT
MicroRNAs (miRNAs) play vital regulatory roles in plant growth and development. The phase transition from vegetative to flowering is crucial the life cycle of plants. To date, miRNA-mediated networks remain largely unexplored radish. In this study, two small RNA libraries radish leaves at reproductive stages were constructed sequenced by Solexa sequencing. A total 94 known miRNAs representing 21 conserved 13 non-conserved miRNA families, 44 potential novel miRNAs, identified libraries. addition, 42 17 significantly differentially expressed as bolting-related miRNAs. RT-qPCR analysis revealed that some exhibited tissue- or developmental stage-specific expression patterns. Moreover, 154 target transcripts for 50 which predominately involved development, signal transduction transcriptional regulation. Based on characterization their genes, a putative schematic model bolting network was proposed. These results could provide insights into radish, facilitate dissecting molecular mechanisms underlying time regulation vegetable crops.
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