Proteomic analysis of endoplasmic reticulum stress responses in rice seeds

• DOI: 10.1038/srep14255
• Publication Date: 2015-09-23T09:07:22Z
• Authors: Dandan Qian; Lihong Tian; Leqing Qu
• Keywords: Endoplasmic-reticulum-associated protein degradation; Proteome; Protein Degradation; Genetically modified rice

Abstract The defects in storage proteins secretion the endosperm of transgenic rice seeds often leads to endoplasmic reticulum (ER) stress, which produces floury and shrunken seeds, but mechanism this response remains unclear. We used an iTRAQ-based proteomics analysis ER-stressed due endosperm-specific suppression OsSar1 identify changes protein levels ER stress. stress changed expression 405 seed by >2.0- fold compared with wild-type control. Of these proteins, 140 were upregulated 265 downregulated. mainly involved modification, transport degradation downregulated metabolism stress/defense responses. A KOBAS revealed that protein-processing degradation-related proteasome predominant pathways Trans-Golgi was also response. Combined bioinformatic molecular biology analyses, our proteomic data will facilitate understanding systemic responses seeds.