Transcriptome and gene expression analysis of DHA producer Aurantiochytrium under low temperature conditions

• DOI: 10.1038/srep14446
• Publication Date: 2015-09-25T08:14:39Z
• Authors: Zengxin Ma; Yanzhen Tan; Guzhen Cui; Yingang Feng; Qiu Cui; Xiaojin Song
• Keywords: RNA-Seq

Abstract Aurantiochytrium is a promising docosahexaenoic acid (DHA) production candidate due to its fast growth rate and high proportions of lipid DHA content. In this study, high-throughput RNA sequencing technology was employed explore the acclimatization producer under cold stress at transcriptional level. The overall de novo assembly cDNA sequence data generated 29,783 unigenes, with an average length 1,200 bp. total, 13,245 unigenes were annotated in least one database. A comparative genomic analysis between normal conditions revealed that 2,013 genes differentially expressed during stage, while 2,071 accumulation stage. Further functional categorization analyses showed some involved processes crucial acclimation, such as signal transduction, cellular component biogenesis carbohydrate metabolism. brief survey transcripts obtained response underlines survival strategy ; these transcripts, many directly or indirectly influence composition. This first study perform transcriptomic low temperature conditions. Our results will help enhance by future.