Kaempferol Identified by Zebrafish Assay and Fine Fractionations Strategy from Dysosma versipellis Inhibits Angiogenesis through VEGF and FGF Pathways

• DOI: 10.1038/srep14468
• Publication Date: 2015-10-08T09:41:16Z
• Authors: Fang Liang; Yuxiang Han; Hao Gao; Shengchang Xin; Shaodan Chen; Nan Wang; Wei Qin; Hanbing Zhong; Shuo Lin; Xinsheng Yao; Song Li
• Keywords: 0301 basic medicine; Embryo, Nonmammalian; Plants, Medicinal; Plant Extracts; Neovascularization, Physiologic; Angiogenesis Inhibitors; Chemical Fractionation; Berberidaceae; Vascular Endothelial Growth Factor Receptor-2; Article; 3. Good health; Animals, Genetically Modified; Fibroblast Growth Factors; Small Molecule Libraries; 03 medical and health sciences; Gene Expression Regulation; Cell Movement; Human Umbilical Vein Endothelial Cells; Animals; Humans; Biological Assay; Kaempferols; Medicine, Chinese Traditional; Cell Proliferation

Abstract Natural products are a rich resource for the discovery of therapeutic substances. By directly using 504 fine fractions from isolated traditional Chinese medicine plants, we performed transgenic zebrafish based screen anti-angiogenesis One fraction, DYVE-D3, was found to inhibit growth intersegmental vessels in vasculature. Bioassay-guided isolation DYVE-D3 indicates that flavonoid kaempferol active substance. Kaempferol also inhibited proliferation and migration HUVECs vitro . Furthermore, suppressed angiogenesis through inhibiting VEGFR2 expression, which can be enhanced by FGF inhibition. In summary, this study shows construction fraction libraries allows efficient identification substances natural products.