Boletus edulis Nitrite Reductase Reduces Nitrite Content of Pickles and Mitigates Intoxication in Nitrite-intoxicated Mice

• DOI: 10.1038/srep14907
• Publication Date: 2015-10-08T11:05:48Z
• Authors: Weiwei Zhang; Guoting Tian; Shanshan Feng; Jack Ho Wong; Yongchang Zhao; Xiao Chen; Hexiang Wang; Tzi Bun Ng
• Keywords: Male; 0301 basic medicine; 0303 health sciences; Nitrite Reductases; Nitrosamines; Sodium Nitrite; Antidotes; Temperature; Hydrogen-Ion Concentration; Article; Diet; 3. Good health; Fungal Proteins; Rats, Sprague-Dawley; Kinetics; Mice; 03 medical and health sciences; Fermentation; Carcinogens; Animals; Humans; Fruiting Bodies, Fungal; Agaricales; Methemoglobin; Enzyme Assays

Pickles are popular in China and exhibits health-promoting effects. However, nitrite produced during fermentation adversely affects health due to formation of methemoglobin conversion carcinogenic nitrosamine. Fruiting bodies the mushroom Boletus edulis were capable inhibiting production pickle fermentation. A 90-kDa reductase (NiR), demonstrating peptide sequence homology fungal reductase, was isolated from B. fruiting bodies. The optimum temperature pH enzyme 45 °C 6.8, respectively. NiR prolonging lifespan nitrite-intoxicated mice, indicating that it had action an antidote. could also eliminate blood after intragastric administration sodium nitrite, packaging into capsule, this nitrite-eliminating activity persist for at least 120 minutes thus avoiding immediate gastric degradation. represents first purified mushrooms may facilitate subsequent applications.