Anodic Electrogenerated Chemiluminescence of Ru(bpy)32+ with CdSe Quantum Dots as Coreactant and Its Application in Quantitative Detection of DNA

• DOI: 10.1038/srep15392
• Publication Date: 2015-10-16T09:10:44Z
• Authors: Yong-Ping Dong; Ting-Ting Gao; Ying Zhou; Li-Ping Jiang; Jun-Jie Zhu
• Keywords: Nucleic Acid Hybridization; Biosensing Techniques; DNA; Electrochemical Techniques; 01 natural sciences; Article; 0104 chemical sciences; 2,2'-Dipyridyl; Coordination Complexes; Limit of Detection; Luminescent Measurements; Quantum Dots; Cadmium Compounds; Selenium Compounds; Electrodes; Oxidation-Reduction

In the present paper, we report that CdSe quantum dots (QDs) can act as coreactant of Ru(bpy)3(2+) electrogenerated chemiluminescence (ECL) in neutral condition. Strong anodic ECL signal was observed at ~1.10 V QDs modified glassy carbon electrode (CdSe/GCE), which might be mainly attributed to apparent electrocatalytic effect on oxidation Ru(bpy)3(2+). intercalated into loop hairpin DNA through electrostatic interaction fabricate a probe. When probe bound GCE, intense obtained. The more when has larger diameter and stronger observed. opened presence target release immobilized Ru(bpy)3(2+), result decrease signal. decreased varied linearly with concentration DNA, showed biosensor used sensitive detection DNA. proposed an excellent performance high specificity, wide linear range low limit.