Metabolomics reveals metabolic changes in male reproductive cells exposed to thirdhand smoke

Viability assay Metabolic pathway
DOI: 10.1038/srep15512 Publication Date: 2015-10-22T09:19:03Z
ABSTRACT
Thirdhand smoke (THS) is a new term for the toxins in cigarette that linger environment long after cigarettes are extinguished. The effects of THS exposure on male reproduction have not yet been studied. In this study, metabolic changes germ cell lines (GC-2 and TM-4) were analyzed treatment 24 h. THS-loaded chromatography paper samples generated laboratory chamber system extracted DMEM. At paper: DMEM ratio 50 μg/ml, viability both was normal, as measured by MTT assay markers cytotoxicity, cycle, apoptosis ROS production normal quantitative immunofluorescence. Metabolomic analysis performed methanol extracts GC-2 TM-4 cells. Glutathione metabolism cells, nucleic acid ammonia changed significantly treatment. RT-PCR analyses mRNA enzyme genes Gss Ggt TK, SMS Glna cells reinforced these findings, showing levels enzymes involved relevant pathways. conclusion, to at very low concentrations caused distinct two different types reproductive lines.
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