Revisiting the reference genomes of human pathogenic Cryptosporidium species: reannotation of C. parvum Iowa and a new C. hominis reference
0301 basic medicine
Amoebiasis: Pathogenesis and Clinical Management
Cryptosporidiosis
Cryptosporidium
Pathogenesis
FOS: Health sciences
Gene
Article
Computational biology
03 medical and health sciences
Diagnosis and Treatment of Prosthetic Joint Infections
Databases, Genetic
Health Sciences
Genetics
Humans
DNA sequencing
Biology
Phylogeny
Immunology and Microbiology
Cryptosporidium parvum
Genome
Bacteria
Comparative genomics
Computational Biology
High-Throughput Nucleotide Sequencing
Life Sciences
Molecular Sequence Annotation
Genomics
Axenic
3. Good health
Molecular Typing
Gene Ontology
Infectious Diseases
Epidemiology and Molecular Characterization of Parasitic Diseases
Whole genome sequencing
FOS: Biological sciences
Medicine
Parasitology
Surgery
Reference genome
Genome, Protozoan
DOI:
10.1038/srep16324
Publication Date:
2015-11-09T10:34:31Z
AUTHORS (9)
ABSTRACT
AbstractCryptosporidium parvumandC. hominisare the most relevant species of this genus for human health. Both cause a self-limiting diarrhea in immunocompetent individuals, but cause potentially life-threatening disease in the immunocompromised. Despite the importance of these pathogens, only one reference genome of each has been analyzed and published. These two reference genomes were sequenced using automated capillary sequencing; as of yet, no next generation sequencing technology has been applied to improve their assemblies and annotations. ForC. hominis, the main challenge that prevents a larger number of genomes to be sequenced is its resistance to axenic culture. In the present study, we employed next generation technology to analyse the genomic DNA and RNA to generate a new reference genome sequence of aC. hominisstrain isolated directly from human stool and a new genome annotation of theC. parvumIowa reference genome.
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