Revisiting the reference genomes of human pathogenic Cryptosporidium species: reannotation of C. parvum Iowa and a new C. hominis reference

• DOI: 10.1038/srep16324
• Publication Date: 2015-11-09T10:34:31Z
• Authors: Juan P. Isaza; Ana Luz Galván; Victor Polanco; Bernice Huang; Andrey V. Matveyev; Myrna G. Serrano; Patricio Manque; Gregory A. Buck; Juan F. Alzate
• Keywords: Cryptosporidium parvum; Axenic; Comparative Genomics

Abstract Cryptosporidium parvum and C. hominis are the most relevant species of this genus for human health. Both cause a self-limiting diarrhea in immunocompetent individuals, but potentially life-threatening disease immunocompromised. Despite importance these pathogens, only one reference genome each has been analyzed published. These two genomes were sequenced using automated capillary sequencing; as yet, no next generation sequencing technology applied to improve their assemblies annotations. For , main challenge that prevents larger number be is its resistance axenic culture. In present study, we employed analyse genomic DNA RNA generate new sequence strain isolated directly from stool annotation Iowa genome.