Revisiting the reference genomes of human pathogenic Cryptosporidium species: reannotation of C. parvum Iowa and a new C. hominis reference

• DOI: 10.1038/srep16324
• Publication Date: 2015-11-09T10:34:31Z
• Authors: Juan P. Isaza; Ana Luz Galván; Victor Polanco; Bernice Huang; Andrey V. Matveyev; Myrna G. Serrano; Patricio Manque; Gregory A. Buck; Juan F. Alzate
• Keywords: 0301 basic medicine; Amoebiasis: Pathogenesis and Clinical Management; Cryptosporidiosis; Cryptosporidium; Pathogenesis; FOS: Health sciences; Gene; Article; Computational biology; 03 medical and health sciences; Diagnosis and Treatment of Prosthetic Joint Infections; Databases, Genetic; Health Sciences; Genetics; Humans; DNA sequencing; Biology; Phylogeny; Immunology and Microbiology; Cryptosporidium parvum; Genome; Bacteria; Comparative genomics; Computational Biology; High-Throughput Nucleotide Sequencing; Life Sciences; Molecular Sequence Annotation; Genomics; Axenic; 3. Good health; Molecular Typing; Gene Ontology; Infectious Diseases; Epidemiology and Molecular Characterization of Parasitic Diseases; Whole genome sequencing; FOS: Biological sciences; Medicine; Parasitology; Surgery; Reference genome; Genome, Protozoan

AbstractCryptosporidium parvumandC. hominisare the most relevant species of this genus for human health. Both cause a self-limiting diarrhea in immunocompetent individuals, but cause potentially life-threatening disease in the immunocompromised. Despite the importance of these pathogens, only one reference genome of each has been analyzed and published. These two reference genomes were sequenced using automated capillary sequencing; as of yet, no next generation sequencing technology has been applied to improve their assemblies and annotations. ForC. hominis, the main challenge that prevents a larger number of genomes to be sequenced is its resistance to axenic culture. In the present study, we employed next generation technology to analyse the genomic DNA and RNA to generate a new reference genome sequence of aC. hominisstrain isolated directly from human stool and a new genome annotation of theC. parvumIowa reference genome.