Analysis of the Secretome of Apoptotic Peripheral Blood Mononuclear Cells: Impact of Released Proteins and Exosomes for Tissue Regeneration

• DOI: 10.1038/srep16662
• Publication Date: 2015-11-16T10:39:35Z
• Authors: Lucian Beer; Matthias Zimmermann; Andreas Mitterbauer; Adolf Ellinger; Florian Gruber; Marie-Sophie Narzt; Maria Zellner; Mariann Gyöngyösi; Sibylle Madlener; Elisabeth Simader; Christian Gabriel; Michael Mildner; Hendrik Jan Anker...
• Keywords: Exosome; Proteome

Abstract We previously showed that, when peripheral blood mononuclear cells (PBMCs) were stressed with ionizing radiation, they released paracrine factors that regenerative capacity in vitro and vivo . This study aimed to characterize the secretome of PBMCs investigate its biologically active components Bioinformatics analysis revealed irradiated differentially expressed genes encoded secreted proteins. These primarily involved (a) pro-angiogenic pathways (b) generation oxidized phospholipids known inflammation-modulating properties. Subsequently, assays exosome protein fractions non-irradiated PBMC major biological enhanced cell mobility; conversely, lipids microparticles had no effects. tested a viral-cleared secretome, prepared according good manufacturing practice (GMP), porcine model closed chest, acute myocardial infarction. found potency for preventing ventricular remodeling was similar GMP-compliant experimentally-prepared secretomes. Our results indicate irradiation modulates release proteins, lipid-mediators extracellular vesicles from human PBMCs. In addition our findings implicate use as valuable material development cell-free therapies medicine.