Cep55 regulates spindle organization and cell cycle progression in meiotic oocyte

• DOI: 10.1038/srep16978
• Publication Date: 2015-11-19T10:25:10Z
• Authors: Zhao-Yang Xu; Xue-Shan Ma; Shu-Tao Qi; Zhen-Bo Wang; Lei Guo; Heide Schatten; Qing-Yuan Sun; Ying-Pu Sun
• Keywords: Germinal vesicle; Spindle pole body; Midbody; Spindle checkpoint; Telophase; Microtubule organizing center; Polar body; Multipolar spindles

Abstract Cep55 is a relatively novel member of the centrosomal protein family. Here, we show that expressed in mouse oocytes from germinal vesicle (GV) to metaphase II (MII) stages. Immuostaining and confocal microscopy as well time lapse live imaging after injection mRNA encoding fusion GFP identified was localized meiotic spindle, especially spindle poles at metaphase, while it concentrated midbody telophase oocytes. Knockdown by specific siRNA caused dissociation γ-tubulin poles, resulting severely defective spindles misaligned chromosomes, leading I arrest failure first polar body (PB1) extrusion. Correspondingly, cyclin B accumulation assembly checkpoint (SAC) activation were observed knockdown Our results suggest may act an MTOC-associated regulating organization thus cell cycle progression during oocyte maturation.